S.O.S. Mathematics CyberBoard

i think im sleepy or confused cuz i dont even understand what im suppose to do

i have to make a standard curve using a 1mg/ml sample of BSA

i have to place 3 ml of Bradford reagent into 8 test tubes

but only 5 of the 8 tubes are going to be used to construct the curve , while 2 will contain 2 unknowns

--- im okay up to here ( its like what would the last test tube be used for , im gonna say a blank so that i could calibrate it based on the Bradford reagent )

i think i forgot how to do these stupid calculations tbh.. its like int he 5 standard test tubes , place 2, 5, 10, 25 and 50 microliters of 1mg/ml of BSA

then it goes on rambling bout stuff i know... cuz i think im just suppose to convert to ml and then do c1v1=c2v2